Huvec Primary Cell Line

Preparing cell culture flasks for culturing huvec.

Huvec primary cell line.

All cells test negative for mycoplasma bacteria yeast and fungi. Warm complete growth medium to 37 c prior to use with the cells. Normal human umbilical vein endothelial cells huvec primary 309 09. Lonza offers a comprehensive range of normal human primary endothelial cells and supporting media for cardiac aortic coronary iliac artery pulmonary artery cardiac microvascular.

Primary human umbilical vein endothelial cells huvec are isolated from the vein of the umbilical cord and are commonly used for physiological and pharmacological investigations such as macromolecule transport blood coagulation angiogenesis and fibrinolysis. They are guaranteed to express cd31 105 and survive in culture through 15 population doublings except huvec xl tm guaranteed through 5 pd. Passage normal huvec cells when culture has reached approximately 80 confluence. Promocell offers huvec isolated in standard endothelial cell growth medium.

Warm both the trypsin edta for primary cells atcc pcs 999 003 and the trypsin neutralizing solution atcc pcs 999 004 to room temperature prior to dissociation. Many of the endothelial processes are commonly studied in vitro using human umbilical vein endothelial cells huvec or large vessel and microvascular endothelial cells isolated from various body tissues. Product overview lonza s cryopreserved huvecs are shipped as frozen primaries. Performance testedat least 16 population doublings when cultured in medium 200 supplemented with low serum growth supplement lsgs guarant.

Passage normal huvec cells when culture has reached approximately 80 confluence. Keep the medium to surface area ratio at 1ml per 5 cm 2. Warm both the trypsin edta for primary cells atcc pcs 999 003 and the trypsin neutralizing solution atcc pcs 999 004 to room temperature prior to dissociation. Human umbilical vein endothelial cells huvec 10 donor pool 233 08.

Normal human umbilical vein endothelial cells huvec primary 309 09. Pipette 20 ml of endothelial cell growth medium to a t 75 flask. Warm complete growth medium to 37 c prior to use with the cells. Primary human umbilical vein endothelial cells huvec are isolated from human umbilical vein and cryopreserved at the end of the primary culture the cells are.