Human Mesenchymal Stem Cell Culture Protocol

Coat a 6well tissue culture plate with 10 μg ml human fibronectin or bovine fibronectin according to the instruction manual.

Human mesenchymal stem cell culture protocol.

Learn more about our hla. Complete portfolio of primary human mesenchymal stem cells hmsc and optimized growth and differentiation media. Osteogenesis of mesenchymal stem cells. Mesenchymal stem cell protocols download a free copy of our gibco mesenchymal stem cell sourcebook for a complete product reference guide product recommendations applications data and publications to support your msc workflow.

When handling biohazard materials such as human cells safe laboratory procedures should be followed and protective clothing should be worn. Cryopreserved poietics tm normal human bone marrow derived mesenchymal stem cells hmsc are guaranteed to have at least 0 75 million viable cells per vial differentiate down the adipogenic chondrogenic and osteogenic liniages when cultured in the recommended differentiation medium to express cd29 cd44 cd105 and cd166 and to not express. Coat the culture vessel. Our hmsc s are available from bone marrow adipose tissue and umbilical cord matrix of individual donors.

Whether mscs are used in basic research or in translational studies establishing the optimal mesenchymal stem cell expansion method and best performing medium allows. Pre warm the completed stemxvivo msc expansion media in a 37 c water bath. Protocol for mesenchymal stem cell expansion mesenchymal stem cells mscs are currently widely used in clinical trials for cell therapy and regenerative medicine applications. This procedure uses 20 ml for each t75 flask used.

Plate mscs at 1x10 5 cells per well in the fibronectincoated tissue culture plate using msc growth medium 2 work in duplicate. Seed mesenchymal stem cells. Culturing of mesenchymal stem cells note.